Bone-Staining Technique for Fetal Rat Specimens without Skinning and Removing Adipose Tissue

P96 Technique for Tissue Plastination in Preparing Long-Lasting Anatomical Specimens

Background: Plastination is a technique of long-term preservation of the natural tissues without the typical problems related to wet specimens. Plastinated specimens are durable, dry, and flexible. In this process, the water and fat in biological tissues are substituted by a curable polymeric resin, i.e., subsequently cured. In this study, a new unsaturated polyester resin (P96) was synthesized...

Cytotoxicity of Chitosan Derived from Shrimp for Bone Scaffold on Adipose Tissue-Derived Mesenchymal Stem Cells

Cellularity of adipose tissue in fetal pig.

Adipose tissue cellularity was studied in the 85-day-old Large-White pig fetus. The aim of this work was to count the adipose cells of forming tissue in an animal species which could be a possible model for studying adipose tissue in humans. Using a morphometric method with electron microscopy, mean triglyceride volume per cell was determined independently of mean cell volume. This method is su...

Fetal bone cells for tissue engineering.

We envision the use of human fetal bone cells for engineered regeneration of adult skeletal tissue. A description of their cellular function is then necessary. To our knowledge, there is no description of human primary fetal bone cells treated with differentiation factors. The characterization of fetal bone cells is particularly important as the pattern of secreted proteins from osteoblasts has...

An autometallographic technique for myelin staining in formaldehyde-fixed tissue.

A new autometallographic (AMG) technique for staining myelin in formaldehyde- or paraformaldehyde- (PFA) fixed tissue is presented. The tissue sections were exposed to AMG development without prior treatment with silver salts. The method was examined on PFA-fixed tissue from mouse, rat, pig, and formaldehyde-fixed human autopsy material. Samples from brain, spinal cord, cranial, and spinal nerv...